Description
Simmons Citrate Agar is used for the differentiation of gram negative bacteria on the basis of citrate utilization.
Organisms able to utilize ammonium dihydrogen phosphate and sodium citrate as the sole sources of nitrogen and carbon, respectively, will grow on this medium and produce an alkaline reaction as evidenced by a change in the color of the bromthymol blue indicator from green (neutral) to blue (alkaline).
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